Journal: iScience

Article Title: DOCK8-expressing T follicular helper cells newly generated beyond self-organized criticality cause systemic lupus erythematosus

doi: 10.1016/j.isci.2021.103537

Figure Lengend Snippet:

Article Snippet: Mouse IL-10 ELISA kit , Biolegend , Cat#431414.

Techniques: Activation Assay, Recombinant, Avidin-Biotin Assay, Plasmid Preparation, Staining, Diagnostic Assay, Magnetic Beads, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Isolation, Purification, Expressing, Microarray

Journal:

Article Title: Involvement of NO in the failure of neutrophil migration in sepsis induced by Staphylococcus aureus

doi: 10.1038/sj.bjp.0704734

Figure Lengend Snippet: Concentration of TNF-α (A and B), IL-1β (C and D), and IL-10 (E and F) in the peritoneal exudate and in sera of mice injected with PBS, sub-lethal or lethal inoculums of S. aureus pre-treated or not with aminoguanidine. The cytokine concentrations in peritoneal exudate (left panels) and in sera (right panels) were determined at 4 h after PBS injection or inoculation of sub-lethal (SL, 0.5×109 CFU/animal), or lethal (L, 16×109 CFU/animal) inoculums of S. aureus pre-treated with PBS or with aminoguanidine (Ag, 30 mg Kg−1, 30 min before bacterial inoculation). Results are expressed as mean±s.e. nanograms of cytokine per millilitre of exudate or sera (n=4). *P<0.01 compared with PBS group. +P<0.05 compared with sublethal inoculum. #P<0.05 compared with lethal inoculum (analysis of variance, followed by Bonferroni's test).

Article Snippet: Drugs, reagents, and antibodies The following materials were obtained from the sources indicated. rmIL-1β (lot 63/668; specific activity, 100,000 IU/0.1 μg ampoule), rmTNF-α (lot 99/532; specific activity, 200,000 IU/1 μg ampoule), purified anti-mouse IL-1β, purified anti-mouse TNF-α, biotinylated anti-mouse TNF-α (lot 250697), and biotinylated anti-mouse IL-1β (lot 250997) were gifts of Dr S. Poole (National Institute for Biological Standards and Control, London, U.K.). rmIL-10 (417-ML), anti-mouse IL-10 monoclonal antibody (MAB417), and biotinylated anti-mouse IL-10 (BAF417) were purchased from R&D Laboratories (Minneapolis, MN, U.S.A.).

Techniques: Concentration Assay, Injection

Journal: PLoS ONE

Article Title: Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51

doi: 10.1371/journal.pone.0136696

Figure Lengend Snippet: Monoclonal antibodies (mAbs) against cell surface markers, intracellular cytokine, nuclear protein or immunoglobulin used in this study.

Article Snippet: The ELISA for detection of IL-10 was performed according Kwong et al. [ ], using anti-bovine IL-10 (clone CC318) (AbD Serotec, USA) as capture antibody, anti-bovine IL-10-biotin (clone CC320) (AbD Serotec, USA) as detection antibody ( ) and recombinant bovine IL-10 (Kingfisher, USA) for developing a standard curve.

Techniques: Binding Assay, Concentration Assay